Characterization and regulation of the expression of scyllatoxin (Leiurotoxin I) receptors in the human neuroblastoma cell line NB‐OK 1

FEBS Letters

Gossen, Denis¹  Christophe, Jean¹  Neef, Philippe De¹  Tastenoy, Michèle¹  Waelbroeck, Magali¹  Gesquière, Jean-Claude² 

[1] Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, B-1000 Brussels, Belgium;Institut Pasteur de Lille, 59019 Lille Cedex, France

关键词: Scyllatoxin (Leiurotoxin I);    Apamin;    Ca2+-activated K+ channel;    Butyrate;    Human neuroblastoma cell line NB-OK 1;    K d;    concentration required for half-maximal occupancy of binding sites with tracer;    1C50;    concentration required for half-maximal inhibition of tracer binding;   

DOI  :  10.1016/0014-5793(91)80815-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

¹²⁵I-[Tyr²]scyllatoxin allowed to label a single class of high-affinity receptors in membranes from the human neuroblastoma cell line NB-OK 1. The K_d of these receptors was 60 pM for scyllatoxin (Leiurotoxin I) and 20 pM for apamin and the B _max was low (3.8 fmol/mg membrane protein). K⁺ increased toxin binding at low concentrations but exerted opposite effects at high concentrations. Ca²⁺, guanidinium and Na⁺ exerted only inhibitory effects on binding. Scyllatoxin binding sites were overexpressed 2.5-fold after a 24-h cell pretreatment with 2 mM butyrate. This effect was suppressed by cycloheximide.

【 授权许可】

Unknown