Dual effects of microwaves on single Ca2+‐activated K+ channels in cultured kidney cells Vero

FEBS Letters

Chemeris, Nicolay K.²  Geletyuk, Vitaliy I.²  Fesenko, Evgeniy E.¹  Kazachenko, Vladimir N.² 

[1] Laboratory of Reception Biophysics, Institute of Cell Biophysics of Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russian Federation;Laboratory of Nerve Cell Biophysics, Institute of Cell Biophysics of Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russian Federation

关键词: Ca2+-activated K+ channel;    Excised membrane patch;    Millimetre microwave;    Kidney cell Vero;   

DOI  :  10.1016/0014-5793(95)00002-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the patch voltage-clamp method, possible effects of millimetre microwaves (42.25 GHz) on single Ca²⁺-activated K⁺ channels in cultured kidney cells (Vero) were investigated. It was found that exposure to the field of non-thermal power (about 100 μW/cm²) for 20–30 min greatly modifies both the Hill coefficient and an apparent affinity of the channels for Ca_i ²⁺. The data suggest that the field alters both cooperativity and binding characteristics of the channel activation by internal Ca²⁺. The effects depend on initial sensitivity of the channels to Ca²⁺ and the Ca²⁺ concentration applied.

【 授权许可】

Unknown