Polymyxin B, a novel inhibitor of red cell Ca2+‐activated K+ channel

FEBS Letters

Peterajová, Elena¹  Pogády, Jozef¹  Varec̆ka, L̆udovít¹ 

[1] Mental Health Research Center of the Institute for Medical Bionics, Psychiatric Hospital, 90218 Pezinok, Czechoslovakia

关键词: Ca2+-activated K+ channel;    Polymyxin B;    Ion channel inhibition;    (Human red cell);   

DOI  :  10.1016/0014-5793(87)81152-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polymyxin B (PXB), a cyclic peptide antibiotic, in concentrations 0.1–3.0 mg/ml (0.08–4.0 mmol/l), inhibited the K⁺ efflux induced by opening of the Ca²⁺-activated K⁺ channel (the Gárdos effect) in intact human red blood cells. The inhibition was observed when the Gárdos effect was elicited by Ca²⁺ in the presence of vandate, or propranolol, in ATP-depleted cells, and in A23187-treated cells. The inhibition of the Gárdos effect is caused neither by the inhibition of the anion channel by PXB nor by the inhibition of Ca²⁺ entry. It can be ascribed to the inhibition of the Ca²⁺-activated K⁺ channel. The mechanism of the inhibition remains to be elucidated.

【 授权许可】

Unknown