Polypeptide constitution of receptors for apamin, a neurotoxin which blocks a class of Ca2+‐activated K+ channels

FEBS Letters

Lazdunski, Michel¹  Hugues, Michel¹  Auguste, Patrick¹ 

[1] Centre de Biochimie, Centre National de la Recherche Scientifique, Parc Valrose, 06034 Nice Cedex, France

关键词: Apamin;    Ca2+-activated K+ channel;    ANBNOS;    N5-azido 2-nitrobenzoyloxysuccinimide;    ANPAA;    succinimidyl ester azidonitrophenyl amino-acetate;    DSS;    disuccinimidyl suberate;    HSAB;    N-hydroxysuccinimidyl 4-azidobenzoate;    SANPAH;    N-succinimidyl 6-(4′-azido 2′-nitrophenylamino) hexanoate;   

DOI  :  10.1016/0014-5793(89)80451-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Affinity labelling experiments with different azido-¹²⁵I-apamin derivatives were carried out to identify polypeptide components of the apamin-sensitive Ca²⁺-activated K⁺ channel in brain and pheochromocytoma cell membranes. Different polypeptides were labelled with different apamin derivatives. The major component has a molecular mass of about 30 kDa but other components at 45, 58 and 86 kDa were also identified. Results obtained with brain membranes on one hand and pheochromocytoma cells on the other were not exactly identical and suggest that there are sub-types of apamin receptors.

【 授权许可】

Unknown