The Na+/H+ exchanger is phosphorylated in human platelets in response to activating agents

FEBS Letters

Pouyssegur, Jacques¹  Sardet, Claude¹  Livne, Avinoam A.¹ 

[1] C.N.R.S., Centre de Biochimie, Université de Nice, Nice O6O34, France

关键词: Platelet;    Thrombin;    Na+/H+ exchanger;    Phosphorylation;    Okadaic acid;   

DOI  :  10.1016/0014-5793(91)80689-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-Thrombin, phorbol esters (PMA) and 1,2-diacylglycerol (DAG), three activators of the amiloride-sensitive Na⁺/H⁺ exchange in human platelets, rapidly increase the intracellular pH and the level of phosphorylation of the Na⁺/H⁺ exchanger protein (NHE1). This stimulatory effect is suppressed by staurosporine, a potent kinase inhibitor, and increased by okadaic acid, a potent inhibitor of phosphatase 1 and 2A. The modulations of NHE1 phosphorylation by these factors correlate well with their effects on platelet pH. Thus, we conclude that in platelets (i) Na⁺/H⁺ exchange is mediated by NHE1. and (ii) platelet activating agents stimulate NHE1 via the modulation of the kinase/phosphatase equilibrium.

【 授权许可】

Unknown