Guanine nucleotides decrease the free [Ca2+] required for secretion of serotonin from permeabilized blood platelets

FEBS Letters

Davidson, Monica M.L.¹  Haslam, Richard J.¹ 

[1] Department of Pathology, McMaster University, Hamilton, Ontario L8N 3Z5, Canada

关键词: Guanine nucleotide;    Calcium;    Thrombin;    Secretion;    Platelet;    pCa;    -log[Ca2+ free];    Gpp(NH)p;    guanyl-5'-yl imidodiphosphate;    GTPγS;    guanosine 5'-O-(3-thiotriphosphate);    GDPβS;    guanosine 5'-O-(2-thiodiphosphate);    1-octadecyl-2-acetyl-G-3-PC;    1-O-octadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine (synthetic platelet-activating factor);   

DOI  :  10.1016/0014-5793(84)81084-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human platelets containing granule-bound [¹⁴C]serotonin were permeabilized, equilibrated at 0°C with ATP and with various Ca²⁺ buffers and guanine nucleotides, and then incubated at 25°C with or without a stimulatory agonist. Ca²⁺ alone induced the ATP-dependent secretion of [¹⁴C]serotonin (50% at a pCa of 5.1) but the sensitivity of secretrion to Ca²⁺ was greatly enhanced by guanine nucleotides [6-fold by 100μM GTP, 100-fold by 100 μM guanyl-5'-yl imidodiphosphate and > 500-fold by 100 μM guanosine 5'-O-(3-thiotriphosphate)] or by stimulatory agonists (10-fold by 2 units thrombin/ml and 4-fold by 1 μM 1-O-octadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine). When both GTP and a stimulatory agonist were added, they had synergistic effects on secretion. Cyclic GMP and GMP acted similarly to GTP. The effects of all these guanine nucleotides were inhibited by guanosine 5'-O-(2-thiodiphosphate), whereas those of stimulatory agonists were not. Our results demonstrate the presence in platelets of guanine nucleotide-dependent and independent mechanisms regulating the sensitivity of secretrion to Ca²⁺.

【 授权许可】

Unknown