Location of dinucleoside triphosphatase in the matrix space of rat liver mitochondria

FEBS Letters

Cameselle, José Carlos²  Sillero, Antonio¹  Bernet, Diego²  Pinto, Rosa Maria² 

[1] Departamento de Bioquimica, Facultad de Medicina, Universidad Autônoma, 28029 Madrid, Spain;Departamento de Bioquimica y Biologia Molecular y Genética, Facultad de Medicina, Universidad de Extremadura, 06080 Badajoz, Spain

关键词: Dinucleoside triphosphatase;    Diadenostine triphosphate;    Dinucleoside polyphosphate;    Mitochondria (rat liver);    Digitonin;    Submitochondrial fraction;    ApnA;    diadenosine 5′;    5‴-P 1;    Pn -n-phosphate;    buffer A;    10 mM Tris-HCl pH 7.5 1 mM EDTA;    1 mg/ml bovine serum albumin;    buffer TS;    5 mM Tris-HCl;    pH 7.5;    0.25 M sucrose;    NpnN;    dinucleoside 5′;    5‴-P 1;    Pn -n-phosphate;    Np3Nase;    dinucleoside triphosphatase (EC 3.6.1.29);    PDE;    phosphodiesterase I/nucleotide pyrophosphatase (EC: 3.1.4. 1/EC 3.6.1.9);   

DOI  :  10.1016/0014-5793(91)80609-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The submitochondrial location of dinucleoside triphosphatase (EC 3.6.1.29). previously shown to be in part associated with mitochondria, has been studied in rat liver. The precipitability and latency of activity in organelle suspensions, and the profile of solubilization by digitonin, were like those of the matrix space marker glutamate dehydrogenase, and differed from those other submitochondrial fractions. This, and the synthesis of diadenosine polyphosphates by mitochondrial aminoacyl-tRNA synthetases, suggest the occurrence of a pathway for the intramitochondrial turnover of diadenosine 5′,5‴-P ¹,P ³-triphosphate (Ap₂A).

【 授权许可】

Unknown