FEBS Letters | |
2‐Nitrosofluorene and N‐hydroxy‐2‐aminofluorene react with the ubiquinone‐reduction center (center N) of the mitochondrial cytochrome bc 1 complex | |
Brandt, Ulrich1  Klöhn, Peter-Christian2  Neumann, Hans-Günter2  | |
[1] Zentrum der Biologischen Chemie, Universität Frankfurt, Frankfurt, Germany;Institut für Toxikologie, Universität Würzburg, Versbacher Str. 9, 97078 Würzburg, Germany | |
关键词: Cytochrome bc 1 complex; Cytochrome b; 2-Nitrosofluorene; N-Hydroxy-2-aminofluorene; Mitochondria (rat liver); AA; antimycin A; AAF; 2-acetylaminofluorene; FCCP; carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone; Myxo; myxothiazol; MOA; E-β-methoxyacrylate stilbene; NBH; nonylubihydroquinone (2; 3-dimethoxy-6-methyl-5-nonylbenzohydroquinone); NOF; 2-nitrosofluorene; N-OH-AF; N-hydroxy-2-aminofluorene; Succ; succinate; TMPD; N; N; N′; N′-tetramethyl-p-phenylenediamine; TTFA; thenoyltrifluoroacetone; | |
DOI : 10.1016/0014-5793(96)00592-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We determined the sites of artificial electron transfer onto 2-nitrosofluorene (NOF), a metabolite of carcinogenic 2-acetylaminofluorene in mitochondria and isolated cytochrome bc 1 complex. NOF-induced O2 consumption in mitochondria was sensitive to antimycin A, but insensitive to myxothiazol. In the isolated cytochrome bc 1 complex, NOF induced rapid MOA-stilbene-insensitive reoxidation of cytochrome b, whereas in the presence of antimycin A, reoxidation was very slow. The corresponding hydroxylamine, N-hydroxy-2-aminofluorene (N-OH-AF), reduced cytochrome b specifically through center N of the cytochrome bc 1 complex. We conclude that NOF and N-OH-AF bind to center N of the cytochrome bc 1 complex and acts as electron acceptor and donor, respectively. The N-OH-AF/NOF interconversion is considered to be involved in the cytotoxicity of 2-acetylaminofluorene in vivo.
【 授权许可】
Unknown
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