Isolation of a novel arachidonic acid metabolite 3‐hydroxy‐5,8,11,14‐eicosatetraenoic acid (3‐HETE) from the yeast Dipodascopsis uninucleata UOFs‐Y128

FEBS Letters

van Dyk, M.S.¹  Coetzee, D.J.¹  Nigam, S.²  Augustyn, O.P.H.³  Kock, J.L.F.¹ 

[1]Department of Microbiology and Biochemistry, University of the Orange Free State, PO Box 339, Bloemfontein, South Africa

[2]Eicosanoid Research, Department of Gynecological Endocrinology, University Medical Centre Steglitz, Free University Berlin, D-1000 Berlin 45, Germany

[3]Vitleultural and Oenological Research Institute, Stellenbosch, South Africa

关键词: Dipodascopsis uninucleata;    3-HETE;    TLC;    HPLC;    EI-MS;    Aspirin-sensitive;   

DOI  :  10.1016/0014-5793(91)80586-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

When arachidonic acid (AA) is added to the yeast Dipodascopsis uninucleata UOFS Y128, one of the major metabolites isolated and purified with the help of thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) is 3-hydroxy-5,8,11,14-eicosatetraenoic acid (3-HETE). The structure of this new AA metabolite was elucidated mainly by electron impact (EI) mass spectrometry (MS). Strikingly, the formation of this new metabolite was found to be inhibited by aspirin.

【 授权许可】

Unknown