Biological dynamics and distribution of 3‐hydroxy fatty acids in the yeast Dipodascopsis uninucleata as investigated by immunofluorescence microscopy. Evidence for a putative regulatory role in the sexual reproductive cycle

FEBS Letters

Nigam, S¹  Kock, J.L.F¹  Schewe, T¹  Linke, D¹  Venter, P¹ 

[1] Eicosanoid Research Division, Department of Gynecology, University Medical Centre Benjamin Franklin, Free University Berlin, D-12200 Berlin, Germany

关键词: AA;    3-HETE;    3-Hydroxy fatty acid;    Immunofluorescence;    Dipodascopsis uninucleata;   

DOI  :  10.1016/S0014-5793(98)00406-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dipodascopsis uninucleata has been recently shown to produce 3-hydroxy polyenoic fatty acids from several exogenous polyenoic fatty acids. In order to examine whether endogenous 3-hydroxy fatty acids (3-OH-FA) may be implicated in the developmental biology of this yeast, we mapped by immunofluorescence microscopy their occurrence in fixed cells with or without cell walls using an antibody raised against 3R-hydroxy-5Z,8Z,11Z,14Z-eicosatetraenoic acid (3R-HETE), the biotransformation product from arachidonic acid (AA). This antibody turned out to cross-react with other 3-OH-FA. 3-OH-FA were detected in situ in gametangia, asci, as well as between released ascospores, and proved to be associated with the sexual reproductive stage of the life cycle of the yeast. Acetylsalicylic acid (1 mM), which is known to suppress the formation of 3-OH-FA from exogenous polyenoic fatty acids, inhibited the occurrence of immunoreactive material as well as the sexual phase of the life cycle suggesting a prominent regulatory role of 3-OH-FA for the latter.

【 授权许可】

Unknown