Adhesive interactions of neutrophils and leukotriene synthesis

FEBS Letters

Barsky, Oleg A.¹  Galkina, Svetlana I.¹  Margolis, Leonid B.¹  Sud'ina, Galina F.¹ 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russian Federation

关键词: 5-Lipoxygenase;    Leukotriene synthesis;    Adhesion;    Human neutrophil;    5-LO;    5-lipoxygenase;    AA;    arachidonic acid;    LTB4;    leukotriene B4;    5S;    12R-dihydroxy-6;    14-cis-8;    10-trans-eicosatetraenoic acid;    5-HETE;    5S-hydroxy-6;    8;    11;    14-eicosatetraenoic acid;    trans-LTB4 isomers;    5S;    12R-dihydroxy-6;    8;    10-trans-14-cis-eicosatetraenoic acid and 5.S;    12S-dihydroxy-6;    8;    10-trans-14-cis-eicosatetraenoic acid;   

DOI  :  10.1016/0014-5793(93)80802-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cell-substrate and cell-cell adhesion of neutrophils has been found to slow down the calcium ionophore A23187-induced synthesis of 5-lipoxygenase (5-LO) metabolites of arachidonic add. Addition of the exogenous substrate, arachidonic acid (AA), together with A23187, resulted in the enhanced production of leukotriene B4 (LTB4) and 5-hydroxyeicosatetraenoic acid (5-HETE) by adherent neutrophils in comparison with those by the cells in suspension. We observed also the enhanced production of 5-LO metabolites in attached cells when we stimulated the cells by the combined action of phorbol 12-myristate 13-acetate (PMA) and A23187. Thus, the adhesion to solid substrate and to other cells, an important regulatory factor for the activity of many cells, is a powerful regulator of leukotriene production by neutrophils.

【 授权许可】

Unknown