Recombinant aequorin as a probe for cytosolic free Ca2+ in Escherichia coli

FEBS Letters

Trewavas, Anthony J.¹  Campbell, Anthony K.²  Knight, Marc R.¹  Smith, Steven M.¹ 

[1] Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3J11, UK;Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff CF4 4XN, UK

关键词: Acquorin reconstitution;    Coelenterazine;    E. coli;    Complement;    Phagocytosis;    Antibiotic;    Cytoplasmic Ca2+;   

DOI  :  10.1016/0014-5793(91)80524-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe a novel and simple method for the measurement of bacterial cytosolic free calcium ([Ca²⁺]_i) using recombinant acquorin reconstituted within live bacterial cells. Using this method we have measured the effects of external calcium, complement, phagocytosis and antibiotics on the [Ca²⁺]_i of Escherichia coli. In principle this method should be applicable to any genetically transformable organism and should suffer fewer problems than fluorescent dyes for subcellular calcium measurement.

【 授权许可】

Unknown