Mass spectrometric evidence for a disulfide bond in aequorin regeneration

FEBS Letters

Fagan, Thomas F.³  Kurono, Sadamu²  Ohashi, Mamoru²  Tsuji, Frederick I.³  Ohmiya, Yoshihiro¹ 

[1] PRESTO, JRDC, Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-01, Japan;Department of Applied Physics and Chemistry, The University of Electro-Communications, Chofu, Tokyo 182, Japan;Osaka Bioscience Institute, 6-2-4 Furuedai, Osaka 565, Japan

关键词: Bioluminescence;    Photoprotein;    Ca2+-binding protein;    Coelenterazine;   

DOI  :  10.1016/0014-5793(93)80637-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tryptic digests of purified recombinant apoaequorin were analyzed, before and after reduction with DTT, by fast atom bombardment mass spectrometry. The results showed that apoaequorin contains a disulfide bond between Cys¹⁴⁵ and Cys¹⁵² and that the reduction of this bond is involved in the regeneration of aequorin.

【 授权许可】

Unknown