Perception of soft mechanical stress in Arabidopsis leaves activates disease resistance

BMC Plant Biology

Research Article

Matteo Binda¹  Eliane Abou-Mansour¹  Lehcen Benikhlef¹  Jean-Pierre Métraux¹  Floriane L’Haridon¹  Mario Serrano¹  Silke Lehmann¹  Alex Costa² 

[1] Department of Biology, University of Fribourg, 10 chemin du Musée, CH-1700, Fribourg, Switzerland;Department of Biosciences, University of Milan, via G. Celoria 26, 20133, Milan, Italy;

关键词: Reactive Oxygen Species;    Potato Dextrose Broth;    Coelenterazine;    Calcofluor White;    Cuticular Permeability;   

DOI  :  10.1186/1471-2229-13-133

 received in 2013-05-15, accepted in 2013-08-01,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundIn a previous study we have shown that wounding of Arabidopsis thaliana leaves induces a strong and transient immunity to Botrytis cinerea, the causal agent of grey mould. Reactive oxygen species (ROS) are formed within minutes after wounding and are required for wound–induced resistance to B. cinerea.ResultsIn this study, we have further explored ROS and resistance to B. cinerea in leaves of A. thaliana exposed to a soft form of mechanical stimulation without overt tissue damage. After gentle mechanical sweeping of leaf surfaces, a strong resistance to B. cinerea was observed. This was preceded by a rapid change in calcium concentration and a release of ROS, accompanied by changes in cuticle permeability, induction of the expression of genes typically associated with mechanical stress and release of biologically active diffusates from the surface. This reaction to soft mechanical stress (SMS) was fully independent of jasmonate (JA signaling). In addition, leaves exposed soft mechanical stress released a biologically active product capable of inducing resistance to B. cinerea in wild type control leaves.ConclusionArabidopsis can detect and convert gentle forms of mechanical stimulation into a strong activation of defense against the virulent fungus B. cinerea.

【 授权许可】

Unknown   
© Benikhlef et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.