Polyphosphoinositides as activators of PKC‐dependent synapsin I phosphorylation

FEBS Letters

Moskvitina, E.L.¹  Severin, S.E.¹ 

[1] Research Center of Molecular Diagnostics, USSR Ministry of Health, Moscow 113149, USSR

关键词: Synapsin I;    Protein kinase C;    Phosphorylation;    Polyphosphoinositide;    Diacylglycerol;    Activation;    SI;    synapsin I;    PKC;    protein kinase C;    PIP2;    phosphatidylinositol-4;    5-bisphosphate;    PS;    phosphatidylserine;    PI;    phosphatidylinositol;    DOG;    dioleoylglycerol;   

DOI  :  10.1016/0014-5793(91)80480-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of PIP₂ and diacylglycerol (products of polyphosphoinositide turnover) on the activation level of phosphorylation of the human brain neurospecific protein SI by PKC from the same source was studied. The apparent activation constant of the phosphorylation process was shown to decrease in the presence of PIP₂ from 1.1 μg/ml to 0.2 μg/ml for PI and from 0.8 μg/ml to 0.6 μg/ml for PS; the value of 0.4 μg/ml in the latter case was detected merely after the addition of DOG into the reaction mixture. Polyphosphoinositides are suggested to play a role in activating PKC-mediated phosphorylation of SI in nerve terminals.

【 授权许可】

Unknown