Cloning and sequence analysis of complementary DNA encoding a precursor for chicken natriuretic peptide

FEBS Letters

Matsou, Hisayuki²  Akizuki, Naoya¹  Minamino, Naoto²  Kangawa, Kenji¹ 

[1] Department of Biochemistryf, Miyazaki Medical College, Kihara, Kiyotake, Miyazaki 889-16, Japan;National Cardiovascular Center Research Institute, Fujishirodai, Suita, Osaka 565, Japan

关键词: Chicken natriuretic peptide;    Atrial natriuretic peptide;    Brain natriuretic peptide;    cDNA cloning;    Chicken heart;    chNP;    chicken natriuretic peptide;    cDNA;    complementary DNA;    ANP;    atrial natriuretic peptide;    BNP;    brain natriuretic peptide;    p.f.u;    plaque forming unit;    Pipes;    piperazine N;    N′-bis(2-ethanesulfonic acid);    bp;    base pairs;    EDTA;    ethylenendiaminetetraacetic acid;    dNTP;    each of dGTP;    dATP;    dCTP;    and dTTP;   

DOI  :  10.1016/0014-5793(91)80331-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chicken α-natriuretic peptide (α-chNP) has been identified in chicken heart, which showed higher homology to brain natriuretic peptide (BNP) than to atrial natriuretic peptide (ANP) [1]. Complementary DNA (cDNA) clone encoding a chNP precursor (pre-chNP) precursor (pre-chNP) was isolated from cardiac cDNA library and sequenced. Pre-chNP was 140-residue signal peptide at the N-terminus and α-chNP at the C-terminus, and did not exhibit high homology to poreine BNP except for the C-terminal region. However, a characteristic AT-rich nucleotide sequence commonly found in mammalian BNPs was also present in the 3′-untranslated region. Thus, chNP is concluded to be classified into the BNP-type

【 授权许可】

Unknown