Brain natriuretic peptide is cosecreted with atrial natriuretic peptide from porcine cardiocytes

FEBS Letters

Nakagawa, Shigeki¹  Takemura, Naoyuki³  Hirata, Yukio²  Iida, Tatsuyoshi¹  Marumo, Fumiaki²  Togashi, Kazumi⁴ 

[1] Department of Biochemistry, Nihon University School of Medicine, Tokyo 173, Japan;Department of Medicine, Tokyo Medical and Dental University, Tokyo 113, Japan;Nippon Veterinary and Zootechnical College, Tokyo 180, Japan;Kitazato Biochemical Laboratories, Sagamihara 228, Japan

关键词: Brain natriuretic peptide;    Atrial natriuretic peptide;    Cosecretion;    Cardiocyte;   

DOI  :  10.1016/0014-5793(90)80076-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using primary cultures of atrial cardiocytes from neonatal pig, the secretion of brain natriuretic peptide (BNP) and atrial natriuretic peptide (ANP)-like immunoreactivities (LI) was studied in vitro. Porcine cardiocytes time-dependently secreted both BNP-LI and ANP-LI into medium under a serum-free condition, although the amount of BNP-LI secreted was about one-third that of ANP-LI. Phorbol ester and Ca²⁺ ionophore had less stimulatory effects on secretion of BNP-LI than that of ANP-LI. Reverse-phase HPLC of the conditioned medium revealed a single major BNP-LI component corresponding to synthetic porcine BNP(1-26). These data suggest that a small molecular weight form BNP, possibly BNP(1-26), is cosecreted with ANP from porcine cardiocytes.

【 授权许可】

Unknown