Brain natriuretic peptide interacts with atrial natriuretic peptide receptor in cultured rat vascular smooth muscle cells

FEBS Letters

Hirata, Yukio²  Kangawa, Kenji³  Marumo, Fumiaki¹  Shichiri, Masayoshi¹  Emori, Toshiaki¹  Matsuo, Hisayuki³ 

[1] Department Of Medicine, Tokyo Medical and Dental University, Tokyo 113, Japan;Hypertension-Endocrine Division, National Cardiovascular Center Research Institute, Osaka 565, Japan;Department of Biochemistry, Miyazaki Medical College, Miyazaki 889-16, Japan

关键词: cyclic GMP;    Brain natriuretic peptide;    Atrial natriuretic peptide;    Receptor binding;    (Vascular smooth muscle cell);   

DOI  :  10.1016/0014-5793(88)80523-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of synthetic porcine brain natriuretic peptide (pBNP), a novel brain peptide with sequence homology to α-human atrial natriuretic peptide (hANP), on receptor binding and cGMP generation, was studied in cultured rat vascular smooth muscle cells (VSMC) and compared with that of α-hANP. ¹²⁵I-pBNP bound to the cells in a time-dependent manner similar to that of ¹²⁵I-α-hANP. Scatchard analysis indicated a single class of binding sites for pBNP with affinity and capacity identical to those of α-hANP. pBNP and α-hANP were almost equipotent in inhibiting the binding of either radioligand and stimulating intracellular cGMP generation. These data indicate that BNP and ANP interact with the same receptor sites to activate guanylate cyclase in rat VSMC.

【 授权许可】

Unknown