Brain natriuretic factor Augmentation of cellular cyclic GMP, activation of particulate guanylate cyclase and receptor binding

FEBS Letters

Song, Dong-Li¹  Kohse, Klaus P.¹  Murad, Ferid¹ 

[1] Departments of Medicine and Pharmacology, Stanford University School of Medicine and Veterans Administration Medical Center, 3801 Miranda Ave, Palo Alto, CA 94304, USA

关键词: Brain natriuretic factor;    Atrial natriuretic factor;    cyclic GMP;    Guanylate cyclase;    Receptor binding;   

DOI  :  10.1016/0014-5793(88)80400-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The newly discovered peptide, brain natriuretic factor (BNF), caused a concentration-dependent increase (up to 400-fold) in intracellular cyclic GMP levels in cultured endothelial, smooth muscle and fibroblast cells. The extent of cGMP augmentation was comparable to that produced by atrial natriuretic factor (ANF). The activity of the membrane-bound guanylate cyclase of different rat tissues and cultured cells was markedly stimulated by the peptide and the addition of ATP potentiated the stimulation. As opposed to tissue particulate guanylate cyclase, the enzyme in cell membranes was slightly more sensitive to activation by BNF than to stimulation by ANF. On bovine aortic smooth muscle (BASM) cells, specific high-affinity binding sites (B _max=398 fmol/10⁶ cells, K _d=0.52 nM) for BNF were observed for which ANF could compete with apparently equal affinity. These results suggest that activation of the cGMP pathways constitutes a common mechanism of action for both BNF and ANF.

【 授权许可】

Unknown