Molecular cloning and expresion of a protein‐tyrosine phosphatase showing homology with transcription factors Fos and Jun

FEBS Letters

Rema, V.¹  Kamatkur, S.¹  Swarup, G.¹  Radha, V.¹ 

[1] Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India

关键词: Protein-tyrosine phosphatase;    cDNA cloning;    Gene expression;    Transcription factor;    PTPase;    Protein-tyrosine phosphatase;    PTP-S;    PTPase cloned from a rat spleen cDNA library;   

DOI  :  10.1016/0014-5793(91)80205-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone coding for a protein-tyrosine phosphatase (PTPase) was isolated from a rat spleen cDNA library. Nucleotide sequence of the clone showed an open reading frame coding for a polypeptide of 363 amino acids. Expression of this clone in E. coli in an expression vector showed PTPase activity. The non-catalytic region of this PTPase located at the carboxy terminus shows homology with the basic domains of transcription factors Fos and Jun. Northern blot analysis showed that a 1.7 kb transcript was present in many tissues and cells, the highest level being in macrophages. This PTPase is a rat homolog of human T-cell PTPase although it shows 3 large deletions in the carboxy terminal non-catalytic region.

【 授权许可】

Unknown