Abundant expression of thromboxane synthase in rat macrophages

FEBS Letters

Tanabe, Tadashi²  Miyata, Atsuro²  Tone, Yoshinori²  Yukawa, Susumu¹  Hara, Shuntaro² 

[1]The 3rd Department of Internal Medicine, Wakayama Medical University, Wakayama 640, Japan

[2]Department of Pharmacology, National Cardiovascular Center Research Institute, Suita, Osaka 565, Japan

关键词: cDNA cloning;    Thromboxane synthase;    Gene expression;    Macrophage;    Rat;    TX;    thromboxane;    PCR;    polymerase chain reaction;    SD;    Sprague-Dawley;    bp;    base pair(s);    kb;    kilobase pair(s);   

DOI  :  10.1016/0014-5793(94)80146-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cloned cDNA for rat thromboxane (TX) synthase with a size of 1851 bp contained a 1599-bp open reading frame which encoded a 533-amino acid protein sharing 79.7% identity with human TX synthase. RNA blot analysis was carried out with rat cells and tissues. Rat peritoneal macrophages most abundantly expressed mRNA for TX synthase, and its level was not changed by in vivo stimulation of casein. Bone marrow, spleen, lung and thymus also expressed the TX synthase gene. These findings suggest the possibility that TXA₂ plays a role in the immune system.

【 授权许可】

Unknown