A 28 kDa mitochondrial protein is radiolabelled by crosslinking with a 123I‐labelled presequence

FEBS Letters

Chich, J.F.²  Thieffry, M.³  Goldschmidt, D.¹  Henry, J.P.²  Gautheron, D.C.¹  Font, B.¹ 

[1] Laboratoire de Biologie et de Technologie des Membranes du CNRS, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France;Service de Neurobiologie Physica-chimique, CNRS, UA 1112, Institut de Biologie Physica-Chimique, 13 rue P. et M. Curie, 75005 Paris, France;Laboratoire de Neurobiologie Cellulaire et Molèculaire, CNRS, 91198 Gif sur Yvette, France

关键词: Addressing sequence;    Mitochondria;    Crosslinking;    peptide M;    MLSLRQSIRFFKY;    EGS;    ethyleneglycol bissuccinimidyl succinate;    CICCP;    carbonylcyanide m-chlorophenyl-hydrazone;   

DOI  :  10.1016/0014-5793(91)80262-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 13-residue peptide containing the first 12 amino acids of the N-terminal part of the signal sequence of yeast cytochrome ???oxidase subunit IV is shown by chemical crosslinking to interact with a mitochondrial protein. This result is obtained with mitochondria from four different origins. Submitochondrial localization experiments suggest that the 28 kDa labelled component is present on the outer face of the inner membrane. Since such addressing peptides are imported into mitochondria through the same machinery as protein precursors, the 28 kDa protein might be a component of the translocation apparatus.

【 授权许可】

Unknown