Association of the glucocorticoid hormone receptor with ribonucleic acid

FEBS Letters

Rousseau, Guy G.¹  Economidis, Ioannis V.¹ 

[1] Hormone and Metabolic Research Unit, University of Louvain and International Institute of Cellular and Molecular Pathology, UCL 7529, Avenue Hippocrate, 75, B-1200 Brussels, Belgium

关键词: Glucocorticoid;    Receptor;    Affinity labeling;    Ribonucleoprotein;    Crosslinking;    dexamethasone;    9α--fluoro-16α-methyl-11β;    17;    21 -trihydroxy-1;    4-pregnadiene-3;    20-dione;    HTC;    hepatoma tissue culture;    Tricine;    N-[2-hydroxy-1;    1-bis-(hydroxymethyl)ethyl]glycine;   

DOI  :  10.1016/0014-5793(85)81111-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hypothesis that the glucocorticoid hormone receptor interacts with RNA has been tested in cultured rat hepatoma cells. The receptor was covalently labeled with radioactive dexamethasone mesylate, and putative RNA-receptor complexes were stabilized by either cell-free crosslinking using formaldehyde or irradiation of intact cells. After chemical cross-linking in vitro, the receptor displayed the buoyant density of a ribonucleoprotein in CsCl gradients. After photochemical crosslinking in cells labeled with radioactive uridine, the receptor analysed by polyacrylamide gel electrophoresis was carrying labeled ribonucleotides.

【 授权许可】

Unknown