In vitro reconstitution of hnRNP particles

FEBS Letters

Kloetzel, Peter M.¹  Schenkel, Johannes² 

[1] Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), INF 282, D-6900 Heidelberg, FRG;University of Heidelberg Medical School, Im Neuenheimer Feld 324, Raum 150, D-6900 Heidelberg, FRG

关键词: hnRNP;    Ribonucleoprotein;    Crosslinking;    Density gradient centrifugation;    hnRNA;    heterogenous nuclear ribonucleic acid;    hnRNP;    heterogenous nuclear ribonucleoprotein particles;    premRNA;    pre-messenger ribonucleic acid;    snRNA;    small nuclear ribonucleic acid;    UV;    ultraviolet;   

DOI  :  10.1016/0014-5793(89)81238-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The assembly of hnRNP-like particles was studied by in vitro reconstitution, UV-crosslinking and CsCl-equilibrium centrifugation. Using total nuclear protein and RNA extracts from HeLa cells for RNP reconstitution, RNP particles sedimenting with the same buoyant density of ϱ=1.4 g/cm³ as ‘native’ 40 S core hnRNPs were obtained. Under the stringent reconstitution conditions used, hnRNP complexes containing only the C1-core hnRNP protein could be identified.

【 授权许可】

Unknown