Effect of different extender on the quality of sexing sperm before freezing in limousin cattle

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Kusumawati, E.D.^1 ; Rahadi, S.^2 ; Isnaini, N.^3 ; Wahjuningsih, S.^3 ; Abdullah, A.G.^4 ; Agustina, R.^5 ; Susilawati, T.^3

Animal Husbandry Study Program, Universitas Kanjuruhan Malang, Jl. S. Supriadi no 48, Malang

65148, Indonesia^1

Animal Science Faculty, Universitas Halu Oleo, Kendari, Indonesia^2

Animal Husbandry Faculty, Brawijaya University, Malang, Indonesia^3

Departemen Pendidikan Teknik Elektro, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi no 229, Bandung

40154, Indonesia^4

Information System Study Program, Universitas Kanjuruhan Malang, Jl. S. Supriadi no 48, Malang

65148, Indonesia^5

关键词: Completely randomized designs;    Cryoprotectants;    Density gradient centrifugation;    Experimental laboratory;    Reaction cells;    research methods;    Shape and size;    Sperm motility;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012102/pdf
DOI  :  10.1088/1757-899X/434/1/012102

来源: IOP

【 摘 要 】

The aim of this study was to determine the quality of sexing sperm before freezing by Percoll density gradient centrifugation method using egg yolks tris aminomethan and andromed® extender. This research method was experimental laboratory studies, the treatment egg yolks tris aminomethan and andromed® extender, parameters observed in the study include motility, percentage of viability and abnormalities fresh sperm before freezing. Sexing results were analyzed using completely randomized design, each treatment consisted of 10 replications. The results showed that both sperm motility and abnormalities using andromed had significant (P