Conservation of engrailed‐like homeobox sequences during vertebrate evolution

FEBS Letters

Holland, Peter W.H.¹  Williams, Nicola A.¹ 

[1] Department of Zoology, University of Oxford, South Parks Road, Oxford, 0X1 3PS, UK

关键词: Engrailed;    Homeobox;    PCR;    Molecular evolution;    Vertebrate development;   

DOI  :  10.1016/0014-5793(90)80858-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Drosophila melanogaster developmental gene engrailed (en) is a member of a distinct subfamily of homeobox genes with a wide phylogenetic distribution. Here we report the use of reduced stringency polymerase chain reaction (PCR) to amplify and clone 8 genes related to en from 5 vertebrate species, including representatives of the most ancient vertebrate lineages. Nucleotide and deduced amino acid sequence comparisons between mouse, toad, zebrafish, lamprey and hagfish genes reveal extensive evolutionary conservation, and suggests that 2 en-like genes have been retained in most vertebrate lineages.

【 授权许可】

Unknown