Sequence analysis of the potent mitogenic toxin of Pasteurella multocida

FEBS Letters

Staddon, James M.¹  Chanter, Neil²  Higgins, Theresa¹  Rozengurt, Enrique¹  Pullinger, Gillian D.²  Lax, Alistair J.² 

[1] Imperial Cancer Research Fund, PO Box 123, Lincoln's Inn Fields, London, WC2A 3PX, UK;AFRC Institute for Animal Health, Compton, Newbury, Berkshire, RG160 NN, UK

关键词: Mitogen;    Toxin;    Protein kinase C;    Pasteurella multocida;   

DOI  :  10.1016/0014-5793(90)80809-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pasteurella multocida toxin is a potent mitogen for cultured Swiss 3T3 cells where it causes an accumulation of inositol phosphates and activation of protein kinase C. The gene sequence described here coded for a 146 kDa protein. The ORF was preceded by a ribosome binding site and followed by a stem loop. There was no evidence for a signal sequence. The gene had a low G + C base ratio which differs from the rest of the Pasteurella genome. There was no significant homology with other known proteins, although a motif found in certain bacterial toxins which are ADP-ribosyl transferases is present. A recombinant expressing only part of the PMT gene was not mitogenic.

【 授权许可】

Unknown