Mutation of a putative ADP‐ribosylation motif in the Pasteurella multocida toxin does not affect mitogenic activity

FEBS Letters

Higgins, Theresa E.²  Mullan, Paul B.¹  Murphy, Anne C.²  Ward, Philip N.¹  Rozengurt, Enrique²  Lax, Alistair J.¹ 

[1] Agricultural and Food Research Council, Institute for Animal Health, Compton, Berkshire, RG16 0NN, UK;Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2A 3PX, UK

关键词: Mitogen;    Toxin;    ADP-ribosylation;    Pasteurella multocida;    Site-directed mutagenesis;    PMT;    Pasteurella multocida toxin;    rPMT;    recombinant Pasteurella multocida toxin;    G protein;    guanine nucleotide binding regulatory protein;    EBL;    embryonic bovine lung;   

DOI  :  10.1016/0014-5793(94)80589-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pasteurella multocida toxin (PMT) is a potent mitogen for Swiss 3T3 fibroblasts and cytotoxic to embryonic bovine lung cells. Site-directed mutagenesis was used to investigate the functional significance of a three amino acid motif in PMT that is present in five other bacterial protein toxins which exhibit ADP-ribosyl transferase activity. Crude lysates of mutant clones were fully cytotoxic for embryonic bovine lung cells. Purified mutant toxin was also as effective at stimulating inositol phosphate turnover and nucleic acid synthesis as wild type toxin. We conclude that this motif has no functional significance in Pasteurella multocida toxin.

【 授权许可】

Unknown