The potent mitogen Pasteurella multocida toxin is highly resistant to proteolysis but becomes susceptible at lysosomal pH

FEBS Letters

Smyth, Martin G.²  Pickersgill, Richard W.¹  Lax, Alistair J.² 

[1] Institute of Food Research, Early Gate, Whiteknights Road, Reading, Berkshire RG6 2EF, UK;Institute for Animal Health, Compton, Berkshire RG16 0NN, UK

关键词: Mitogen;    Toxin;    Pasteurella multocida;    Limited proteolysis;    PMT;    Pasteurella multocida toxin;    G protein;    guanine nucleotide binding regulatory protein;    CTAB;    cetyltrimethylammonium bromide;    RME;    receptor mediated endocytosis;   

DOI  :  10.1016/0014-5793(95)00077-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The susceptibility of the potent mitogen Pasteurella multocida toxin (PMT) to various proteases was investigated. PMT at a toxin to protease molar ratio of 1:1 was resistant to 8 of the 11 proteases tested after one hour. With longer incubation, PMT remained resistant to 7 proteases, and this correlated with a retention of biological activity, indicating that PMT might not require proteolytic cleavage at least until it bound to a cell receptor. Previous evidence had suggested that PMT is processed in the cell via an endosome or lysosome. We have shown that PMT became susceptible to proteolysis when the pH was lowered to 5 or below. This supports the previous suggestion that PMT is processed via a low pH compartment in the cell.

【 授权许可】

Unknown