Chromatin structure of Drosophila melanogaster ribosomal genes

FEBS Letters

Karpov, V.L.¹  Mirzabekov, A.D.¹  Preobrazhenskaya, O.V.¹  Belikov, S.V.¹  Dzherbashyajan, A.R.¹ 

[1] W.A. Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow 117984, USSR

关键词: DNA-protein crosslinking;    Chromatin structure;    Ribosomal gene;    D. melanogaster;   

DOI  :  10.1016/0014-5793(90)81085-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The chromatin structure of ribosomal genes of D. melanogaster has been studied by crosslinking proteins to DNA. We found that a number of histone contacts with DNA through histidine in the approximately 1 kb-long region surrounding the transcription initiation site, coding regions and the region of 240 bp-long repeats from the intergenic spacers (Alu-repeats) were weakened as compared to the inactive chromatin of the type II insertion. A protein with the molecular mass of 50 kDa (p50), associated with all DNA sequences analysed, has been discovered. Another protein with molecular mass of about 70 kDa (p70) has been found to be specific only for the Alu-repeats.

【 授权许可】

Unknown