Mapping of DNA‐binding proteins along the yeast genome by UV‐induced DNA‐protein crosslinking

FEBS Letters

Karpov, V.L.¹  Papatsenko, D.A.¹  Priporova, I.V.¹  Belikov, S.V.¹ 

[1] W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 117984 Moscow, Russia

关键词: UV-light;    DNA-protein crosslinking;    Saccharomyces cerevisiae;    DNA-binding protein;    UV (light);    ultra-violet (light);    TKL2;    transketolase gene;    CMD1;    calmodulin gene;    ALG1;    β-mannosyl transferase gene;    Mm;    molecular mass;    PAAG;    polyacrylamide gel;    2D;    two-dimensional;   

DOI  :  10.1016/0014-5793(96)00091-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

UV-induced crosslinking of DNA-binding proteins to DNA in intact nuclei of Saccharomyces cerevisiae and subsequent ‘protein image’ hybridization were applied to map non-histone proteins along single-copy genes of yeast. We detected two polypeptides that most probably correspond to core subunits of yeast RNA-polymerase II in the coding region of transketolase gene (TKL2). Several non-histone proteins were also detected which bind to the upstream region of TKL2 gene, and to the intergenic spacer between calmodulin (CMD1) and β-mannosyl transferase (ALG1) genes.

【 授权许可】

Unknown