Identification of DNA binding proteins in vaccinia virus by DNA‐protein crosslinking

FEBS Letters

Karpov, V.L.²  Mirzabekov, A.D.²  Prikhod'ko, G.G.¹  Vassilenko, S.K.¹  Chkheidze, A.N.² 

[1] All-Union Institute of Molecular Biology, Scientific-Industrial Association ‘vector’, Koltzovo, Novosibirsk Region 633159, Russian Federation;W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov St. 32, Moscow 117984, Russian Federation

关键词: Vaccinia virus (VV);    Strain LIVP;    DNA binding protein;    DNA-protein crosslinking;    Dimethyl sulfate (DMS);   

DOI  :  10.1016/0014-5793(93)80833-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA binding proteins of vaccinia virus (VV) virions, strain LIVP, have been studied by their covalent crosslinking to DNA, using two-dimensional gel retardation electrophoresis of crosslinked DNA and proteins as well as the ‘protein image’ hybridization assay. Five proteins with molecular masses of 16, 25, 27, 41 and 54 kDa, respectively, associated with all analysed DNA sequences, including early and late genes and their promoters, have been identified.

【 授权许可】

Unknown