The three carboxy‐terminal amino acids of human interleukin‐6 are essential for its biological activity

FEBS Letters

Lütticken, Claudia¹  Freyer, Peter¹  Dufhues, Gabi¹  Krüttgen, Alex¹  Heinrich, Peter C.¹  Bender, Sven¹  Rose-John, Stefan¹ 

[1] Department of Biochemistry, RWTH Aachen, Klinikum, Pauwelsstrasse 30, 5100 Aachen, FRG

关键词: Interleukin-6;    Deletion mutant;    PCR;    Carboxy-terminus;    Biological activity;    Acute phase protein induction;    IL-6;    interleukin-6;    PCR;    polymerase chain reaction;    rh;    recombinant human;   

DOI  :  10.1016/0014-5793(90)81059-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have constructed on the cDNA level deletion mutants of human interleukin-6 lacking one, two, three or four amino acids from the carboxyterminus of the molecule. After in vitro transcription and translation the biological activity of these deletion mutants was determined by two independent bioassays. Both, the mouse B9 cell proliferation assay and the fibrinogen induction assay with the human hepatoma cell line HepG2 led to the following result: already the removal of the last amino acid resulted in a five-fold loss of biological activity. An additional slight reduction was seen when two amino acids were removed from the carboxy-terminus. Interleukin-6 lacking three or four C-terminal amino acids were completely inactive. The presented results emphasize the extreme importance of the carboxy-terminus of interleukin-6 for its biological function.

【 授权许可】

Unknown