期刊论文详细信息
FEBS Letters
Efficient phage display of polypeptides fused to the carboxy‐terminus of the M13 gene‐3 minor coat protein
Sidhu, Sachdev S.1  Fuh, Germaine1 
[1] Department of Protein Engineering, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA
关键词: M13 bacteriophage;    Carboxy-terminus;    Phage display;    Minor coat protein;    P3;    protein-3;    the gene-3 minor coat protein of M13 phage;    P8;    protein-8;    the gene-8 major coat protein of M13 phage;    truncated P3;    P3 residues 257–406;   
DOI  :  10.1016/S0014-5793(00)01946-3
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

We report that, contrary to common belief, polypeptides fused to the carboxy-terminus of the M13 gene-3 minor coat protein are functionally displayed on the phage surface. In a phagemid display system, carboxy-terminal fusion through optimized linker sequences resulted in display levels comparable to those achieved with conventional amino-terminal fusions. These findings are of considerable importance to phage display technology because they enable investigations not suited to amino-terminal display, including the study of protein–protein interactions requiring free carboxy-termini, functional cDNA cloning efforts, and the display of intracellular proteins.

【 授权许可】

Unknown   

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