FEBS Letters | |
Efficient phage display of polypeptides fused to the carboxy‐terminus of the M13 gene‐3 minor coat protein | |
Sidhu, Sachdev S.1  Fuh, Germaine1  | |
[1] Department of Protein Engineering, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA | |
关键词: M13 bacteriophage; Carboxy-terminus; Phage display; Minor coat protein; P3; protein-3; the gene-3 minor coat protein of M13 phage; P8; protein-8; the gene-8 major coat protein of M13 phage; truncated P3; P3 residues 257–406; | |
DOI : 10.1016/S0014-5793(00)01946-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We report that, contrary to common belief, polypeptides fused to the carboxy-terminus of the M13 gene-3 minor coat protein are functionally displayed on the phage surface. In a phagemid display system, carboxy-terminal fusion through optimized linker sequences resulted in display levels comparable to those achieved with conventional amino-terminal fusions. These findings are of considerable importance to phage display technology because they enable investigations not suited to amino-terminal display, including the study of protein–protein interactions requiring free carboxy-termini, functional cDNA cloning efforts, and the display of intracellular proteins.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020309740ZK.pdf | 91KB | download |