Norepinephrine‐induced synthesis of the uncoupling protein thermogenin (UCP) and its mitochondrial targeting in brown adipocytes differentiated in culture

FEBS Letters

Rehnmark, Stefan¹  Herron, David¹  Lončar, Dragutin¹  Néchad, Myriam¹  Cannon, Barbara¹  Nedergaard, Jan¹ 

[1] The Wenner-Gren Institute, The Arrhenius Laboratories F3, Stockholm University, S-106 91 Stockholm, Sweden

关键词: Uncoupling protein;    Thermogenin;    Norepinephrine;    Mitochondrial targeting;    Nonshivering thermogenesis;    Brown adipose tissue;   

DOI  :  10.1016/0014-5793(90)81031-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthesis of the brown adipocyte-specifie mitochondrial uncoupling protein thermogenin (UCP) is demonstrated here in brown adipocytes differentiated in culture from precursor cells. By immunoblotting, no UCP was detectable in untreated multilocular adipocytes. The synthesis of UCP was stimulated by norepinephrine at physiological concentrations and was observable already after 2 h. It was evident from immunoelectron microscopy that the newly synthesised protein was targeted to the mitochondrial inner membrane, demonstrating the functional competence of these cultured cells.

【 授权许可】

Unknown