Exclusive occurrence of thermogenin antigen in brown adipose tissue

FEBS Letters

Cannon, Barbara²  Nedergaard, Jan²  Hedin, Anders¹ 

[1] Department of Immunology, The Wenner-Gren Institute, University of Stockholm, Norrtullsgatan 16, 113 45 Stockholm, Sweden;Department of Metabolic Research, The Wenner-Gren Institute, University of Stockholm, Norrtullsgatan 16, 113 45 Stockholm, Sweden

关键词: Brown adipose tissue;    ELISA;    Mitochondria;    Nonshivering thermogenesis;    Thermogenin;    White adipose tissue;   

DOI  :  10.1016/0014-5793(82)81319-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thermogenin is the purine-nucleotide binding polypeptide in brown adipose tissue mitochondria (M _r 32 000) which confers upon these mitochondria the ability to produce heat. An enzyme-linked immunosorbent assay (ELISA) has been developed to demonstrate and quantitate the occurrence of thermogenin antigen in small amounts of tissue, and thus to characterize different depots of fat tissue as white or brown. The extreme sensitivity of the method allows determination of thermogenin in samples equivalent to <1 mg tissue. The results indicate that thermogenin seems to be exclusively localised in brown fat mitochondria (as compared to white fat, liver or heart muscle mitochondria), and thermogenin antigen could only be found in brown adipocytes (as compared to white adipocytes). Thus, brown and white adipose tissue are probably ontogenetically different

【 授权许可】

Unknown