FEBS Letters | |
α1‐Adrenergic stimulation of Cl− efflux in isolated brown adipocytes | |
Connolly, Eamonn1  Dasso, Leonardo1  Nedergaard, Jan1  | |
[1] The Wenner-Gren Institute, University of Stockholm, The Arrhenius Laboratories F3, S-106 91 Stockholm, Sweden | |
关键词: Brown adipose tissue; Cl− flux; Membrane depolarization; Nonshivering thermogenesis; Prazosin; (Hamster); | |
DOI : 10.1016/0014-5793(90)80144-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Unidirectional 36Cl− efflux from preloaded isolated brown adipocytes was studied. A norepinephrine-stimulated 36Cl− efflux pathway was found which approximately doubled the rate of 36Cl− efflux from the cells. The response to norepinephrine was fully inhibited by the α1-adrenergic antagonist prazosin, but was unaffected by the β-adrenergic antagonist propranolol, showing that norepinephrine stimulated the 36Cl− efflux pathway via the α1-adrenoceptor. The stimulation of 36Cl− efflux could not be mimicked by the Ca2+ ionophore A23187, indicating that the effect was not mediated by elevation of the intracellular Ca2+ level. It is concluded that brown fat cells possess a specific mechanism for α1-adrenergic stimulation of Cl− efflux. The possibility is discussed that this Cl− efflux pathway could be the basis for the early α-adrenergic depolarization seen in brown fat cells.
【 授权许可】
Unknown
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