NADPH:O2 oxidoreductase of human eosinophils in the cell‐free system

FEBS Letters

Stokman, Petra M.¹  Koenderman, Leo¹  Bolscher, Ben G.J.M.¹  Roos, Dirk¹  Tool, Anton T.J.¹ 

[1] Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, Amsterdam, The Netherlands

关键词: Eosinophil;    NADPH oxidase;    Cell-free system;    CGD.chronic granulomatous disease;    Hepes;    N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid;    PMA;    phorbol myristic acid;    STZ;    serum-treated zymosan;    Moab;    monoclonal antibody;    Xb−;    X-linked cytochrome b 558-negative;    Ab+;    autosomal cytochrome b 588-positive;   

DOI  :  10.1016/0014-5793(90)81025-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The NADPH oxidase of human eosinophils, measured in the cell-free system, shows the same characteristics as the enzyme from human neutrophils. All proteins required for activity of the enzyme are expressed in eosinophils at a higher level than in neutrophils. Eosinophils isolated from patients with chronic granulomatous disease show the same molecular defects as the neutrophils fom these patients.

【 授权许可】

Unknown