An edeine resistant mRNA‐dependent protein synthesis system from a Saccharomyces cerevisiae mutant

FEBS Letters

Herrera, Flor¹  Moreno, Nancy¹  Martínez, José Antonio² 

[1] Centro de Investigations Biomédicas, Facultad de Ciencias de la Salud, Universidad de Carabobo, CaraboboVenezuela;Departamento de Biología, Instituto Pedagógico de Maracay, Maracay, Venezuela

关键词: Cell-free system;    Saccharomyces cerevisiae;    Edeine-resistant mutant;    Initiation protein synthesis;   

DOI  :  10.1016/0014-5793(84)81075-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cell-free protein synthesizing system from a mutant of Saccharomyces cerevisiae translated exogenous mRNA in the presence of 2μM edeine, while a similar system from wild-type strain was completely inhibited by the drug. The mutant ribosomes showed an affinity for [¹²⁵I]edeine comparable to the wild-type ribosomes, thereby suggesting that these macromolecules alone were not responsible for the edeine-resistant capacity of the mutant.

【 授权许可】

Unknown