Translation arrest of potato virus X RNA in Krebs‐2 cell‐free system: RNase H cleavage promoted by complementary oligodeoxynucleotides

FEBS Letters

Karpova, Olga V.¹  Morozov, Sergei Yu.¹  Atabekov, Joseph G.¹  Rodionova, Nina P.¹  Miroshnichenko, Nadezhda A.¹ 

[1] Institute of Microbiology, Moscow 117811 USSR and Department of Virology, Moscow State University, Moscow 119899, USSR

关键词: Translational arrest;    Cell-free system;    RNase H cleavage;    Plant virus RNA;   

DOI  :  10.1016/0014-5793(88)81304-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Translation arrest of genomic potato virus X (PVX) RNA promoted by complementary oligodeoxynucleotides in Krebs-2 cell-free system is described. 14–15 mer oligodeoxynucleotides complementary to the 5′-proximal cistron of PVX RNA were shown to induce specific truncation of the major non-structural polypeptide coded by PVX RNA. Evidence is presented that effective translational arrest of PVX RNA in the presence of complementary oligonucleotides restults from the site-specific cleavage of RNA by endogenous RNase H intrinsic to the Krebs-2 extract. No similar translational arrest was found in the rabbit reticulocyte lysate cell-free system.

【 授权许可】

Unknown