Cleavage of lamin‐like proteins in in vivo and in vitro apoptosis of tobacco protoplasts induced by heat shock

FEBS Letters

Zhou, Jun¹  Chen, Hao-Ming¹  Dai, Yao-Ren¹ 

[1] Department of Biology, Tsinghua University, Beijing 100084, PR China

关键词: Lamin-like protein;    Heat shock;    Tobacco protoplast;    Apoptosis;    Cell-free system;    DAPI;    4;    6-diamidino-2-phenylindole;    TUNEL;    terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling;    HS;    heat shock;    PCD;    programmed cell death;   

DOI  :  10.1016/S0014-5793(00)01912-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Apoptosis in heat shock-treated tobacco protoplasts was evidenced by DNA fragmentation, flow cytometric analysis and activation of caspase 3-like protease. Furthermore, an in vitro apoptosis system was established which reproduced the apoptotic events. Western blotting analysis using an antibody against lamin A and C showed that in both in vivo and in vitro systems lamin-like proteins were cleaved into a 35-kDa fragment, and that lamin-like protein degradation precedes DNA fragmentation. Moreover, we found a 22.8-fold increase in caspase 6-like activity in cytosol of heat-treated protoplasts as compared with the control.

【 授权许可】

Unknown