GTP‐dependent and ‐independent activation of superoxide producing NADPH oxidase in a neutrophil cell‐free system

FEBS Letters

Minakami, Shigeki¹  Takasugi, Shin-ichiro¹  Takeshige, Koichiro¹  Ishida, Kiyoshi¹ 

[1] Department of Biochemistry, Kyushu University School of Medicine, Fukuoka 812, Japan

关键词: Superoxide;    NADPH oxidase;    SDS;    Cell-free system;    GTP;    (Pig neutrophil);    GTP-γ-S;    guanosine 5′-O-(3-thiotriphosphate);   

DOI  :  10.1016/0014-5793(89)80123-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

GTP and GTP-γ-S enhanced several-fold the NADPH-dependent superoxide production induced by sodium dodecyl sulfate in a cell-free system of pig neutrophils consisting of the membrane fraction and two cytosolic fractions separated by gel filtration. The enhanced activity was decreased by the addition of GDP in a dose-dependent manner, but 70% of the activity in the absence of GTP remained even at 1 mM GDP. Only one cytosol fraction besides the membrane fraction was required for the activation in the presence of GTP. The cytosol fraction was analyzed by chromatography on 2′,5′-ADP agarose and two components responsible for the GTP-dependent and independent activation were separated. These results suggest that at least two pathways are available for the activation of superoxide production in the cell-free system of pig neutrophils.

【 授权许可】

Unknown