Purification of the 260 kDa cytosolic complex involved in the Superoxide production of guinea pig neutrophils

FEBS Letters

Nagaoka, Isao¹  Someya, Akimasa¹  Yamashita, Tatsuhisa¹ 

[1] Department of Biochemistry, Juntendo University, School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Superoxide production;    NADPH oxidase;    Cytosolic factor;    Neutrophil;    Cell-free system;    p47phox;    47 kDa cytosolic phagocyte oxidase component;    p67phox;    67 kDa cytosolic phagocyte oxidase component;    PMSF;    phenylmethanesulfonyl fluoride;   

DOI  :  10.1016/0014-5793(93)80276-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 260 kDa cytosolic complex (SP-1) was purified from guinea pig neutrophils. SP-1 was composed of 63 kDa, 47 kDa and 39 kDa proteins. The 63 kDa and 47 kDa proteins proved to correspond to human p67^phox and p47^phox by Western blot analysis, whereas Western blot and amino acid sequence analyses revealed that the 39 kDa protein was a novel protein. The 47 kDa protein was separated from the 63 kDa and 39 kDa proteins by dithiothreitol (DTT)-treatment. On the other hand, the 63 kDa and 39 kDa proteins were not separated with DTT, detergent and ethanol treatment. These results suggest that the 39 kDa protein tightly associates with the 63 kDa protein and may regulate the function of the 63 kDa protein.

【 授权许可】

Unknown