Effect of ATP on the activity of bovine heart mitochondrial b‐c 1 complex

FEBS Letters

Lorusso, Michele¹  Cocco, Tiziana¹  Minuto, Michele¹  Papa, Sergio¹ 

[1] Institute of Medical Biochemistry and Chemistry, University of Bari, Bari, Italy

关键词: Mitochondria;    b-c 1 Complex;    Electron transport;    ATP;    Complexing agent;    PMF;    electrochemical proton gradient (proton motive force);    DQH2;    durohydroquinone (duroquinol);   

DOI  :  10.1016/0014-5793(90)80299-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of ATP on the reductase activity of purified bovine heart b-c ₁ complex was studied. ATP stimulates the steady-state activity and the antimycin-insensitive pre-steady-state reduction of b and c ₁ cytochromes, also causing changes of kinetic properties of the enzyme. There is no absolute specificity for ATP since other polyvalent anions such as EDTA and EGTA produce similar effects in the micromolar range. It is proposed that ATP stimulates the activity of the b-c ₁ complex, chelating inhibitory cation(s), exerting a modulatory action on the enzyme.

【 授权许可】

Unknown