The H+/O ratio of proton translocation linked to the oxidation of succinate by mitochondria

FEBS Letters

Lehninger, Albert L.³  Hendler, Richard W.¹  Shrager, Richard I.²  Reynafarje, Baltazar³ 

[1] Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, NIH, Building 3, Room B 1-22, Bethesda, MD 20205, USA;Laboratory of Applied Studies, Division of Computer Research and Technology, NIH, Bethesda, MD 20205, USA;Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725. N. Wolfe Street, Baltimore, MD 21205, USA

关键词: Proton;    Stoichiometry;    Electron transport;    Mitochondria;    Succinate;   

DOI  :  10.1016/0014-5793(85)80102-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Costa, L.E., Reynafarje, B. and Lehninger, A.L. [(1984) J. Biol. Chem. 259, 4802-4811] have reported ‘second-generation’ measurements of the H⁺/O ratio approaching 8.0 for vectorial H⁺ translocation coupled to succinate oxidation by rat liver mitochondria. In a Commentary in this Journal [Krab, K., Soos, J. and Wikström, M. (1984) FEBS Lett. 178, 187-192] it was concluded that the measurements of Costa et al. significantly overestimated the true H⁺/O stoichiometry. It is shown here that the mathematical simulation on which Krab et al. based this claim is faulty and that data reported by Costa et al. had already excluded the criticism advanced by Krab et al. Also reported are new data, obtained under conditions in which the arguments of Krab et al. are irrelevant, which confirm that the H⁺/O ratio for succinate oxidation extrapolated to level flow is close to 8.

【 授权许可】

Unknown