Divergent changes of chromogranin A/secretogranin II levels in differentiating human neuroblastoma cells

FEBS Letters

Påhlman, Sven¹  Floor, Eric²  Weiler, Rosad³  Laslop, Andrea³  Fischer-Colbrie, Reiner³  Meyerson, Gabrielle¹  Winkler, Hans³ 

[1] Department of Pathology, University of Uppsala, Uppsala, Sweden;Anatomy Department, University of Wisconsin, Madison, WI, USA;Department of Pharmacology, University of Innsbruck, Peter-Mayr-Straße 1a, A-6020 Innsbruck, Austria

关键词: Neuroblastoma;    Chromogranin A;    Secretogranin II;    Synaptin/synaptophysin;    SV2;   

DOI  :  10.1016/0014-5793(90)80875-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human neuroblastoma cells were cultured either in the absence or presence of 12-O-tetradecanoylphorbol-13-acetate (TPA) known to induce neuronal differentiation. This treatment led to a marked increase in the concentration of secretogranin II but to a decrease of chromogranin A. Analogous changes were observed for the respective mRNAs. Thus during differentiation of these cells the biosynthesis of two vesicle constituents of large dense core vesicles is differentially regulated as determined both at the mRNA and the protein level. Levels of both synaptin/synaptophysin and SV2 were also elevated but to a smaller degree than that of secretogranin II.

【 授权许可】

Unknown