Palytoxin acidifies chick cardiac cells and activates the Na+/H+ antiporter

FEBS Letters

Frelin, Christian¹  Vigne, Paul¹  Breittmayer, Jean-Philippe² 

[1] Institut de Pharmacologie Moléculaire et Cellulaire, UPR 411, CNRS, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France;Institut de Pharmacologie Moléculaire et Cellulaire, U.210 INSERM, Chemin de Valombrose, 06034 Nice cedex, France

关键词: Palytoxin;    Na+/H+ antiporter;    Amiloride;    Intracellular pH;    PTX;    palytoxin;    EIPA;    ethylisopropylamiloride;    BCECF;    biscarboxyethylcarboxyfluorescein;    pHi;    intracellular pH;    pHo;    extracellular pH;    NMG;    N-methyl-D-glucamine;   

DOI  :  10.1016/0014-5793(90)80765-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cardiotoxic action of palytoxin was investigated using embryonic chick ventricular cells. Under normal ionic conditions, palytoxin produced an intracellular acidification which is partially compensated for by the Na⁺/H⁺ antiporter thereby leading to an increased rate of ethylisopropylami-loride-sensitive ²²Na⁺ uptake. Under depolarizing membrane conditions, palytoxin produced a cellular acidification, a cellular alkalinization or no change in intracellular pH depending on the value of the extracellular pH. We propose that palytoxin acidifies cardiac cells by opening preexisting H⁺ conducting pathways in the plasma membrane.

【 授权许可】

Unknown