Antibodies to the β‐amyloid peptide cross‐react with conformational epitopes in human fibrinogen subunits from peripheral blood

FEBS Letters

Lee, Virginia M.-Y.¹  Stem, Robert A.¹  Trojanowski, John Q.¹ 

[1] The Department of Pathology and Laboratory Medicine, Division of Anatomic Pathology, The University of Pennsylvania School of Medicine, Philadelphia, PA, USA

关键词: Alzheimer's disease;    A4 protein;    β-Amyloid precursor;    Fibrin;    Plasma;   

DOI  :  10.1016/0014-5793(90)80760-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies to the Alzheimer disease (AD) β-amyloid peptide (βAP) were used to identify βAP precursor fragments in blood. The antibodies detected 3 major polypeptides with apparent molecular weights (MW) of 47-64000 in Western blots of plasma derived clot proteins, but these proteins corresponded to human A-α, B-β and γ-fibrinogen since they reacted with 2 different anti-fibrinogen antisera, and the anti-βAP and anti-fibrinogen antibodies recognized purified fibrinogen and fibrin. These data are significant for efforts to develop immunochemical assays to diagnose and monitor the progression of AD.

【 授权许可】

Unknown