Evidence that chlorpromazine and prostaglandin E1 but not neomycin interfere with the inositol phospholipid metabolism in intact human platelets

FEBS Letters

Frølich, Karin W.¹  Tysnes, Ole-Bjørn¹  Holmsen, Holm¹  Steen, Vidar M.¹ 

[1] Department of Biochemistry, University of Bergen, Årstadveien 19, N-5009 Bergen, Norway

关键词: Neomycin;    Chlorpromazine;    Prostaglandin E1;    Signal transduction;    Inositol phospholipid;    Thrombin;    Platelet;    PA;    phosphatidic acid;    PIP;    phosphatidylinositol 4-phosphate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    PGE1;    prostaglandin E1;    PPI;    polyphosphoinositide;   

DOI  :  10.1016/0014-5793(90)80757-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human platelets that had been prelabelled with [³²P]P_i were stimulated with trombin in the presence or absence of neomycin, prostaglandin E₁ (PGE₁) or chlorpromazine. The content of [³²P]P_i in phosphatidylinositol 4-phosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidic acid (PA) were determined. The data demonstrate that PGE₁ and chlorpromazine but not neomycin interfere with the tight metabolic relationship that exists between the inositol phospholipids and PA in thrombin-stimulated platelets [(1989) Biochem. J. 263, 621-624]. Our results therefore indicate that neomycin does not inhibit signal transduction in intact platelets at the level of the inositol phospholipid metabolism.

【 授权许可】

Unknown