The fluorescence response of chlortetracycline‐loaded human neutrophils is modulated by prostaglandin E1, but not by cyclic nucleotides

FEBS Letters

Smolen, James E.¹ 

[1] Division of Pediatric Hematology/Oncology, University of Michigan Medical School, F6515 Mott Children's Hospital, Ann Arbor, MI 48109, USA

关键词: Fluorescence;    Chlortetracycline;    Neutrophil;    Prostaglandin E1;    Cyclic nucleotide;    CTC;    chlortetracycline;    fMet—Leu—Phe;    N-formylmethionyl-leucyl-phenylalanine;   

DOI  :  10.1016/0014-5793(83)81176-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human neutrophils preloaded with chlortetracycline, commonly used as a probe of membrane-bound calcium, demonstrate a prompt decrease in fluorescence when exposed to surface stimuli such as the chemotactic peptide fMet—Leu—Phe. The fluorescence response was highly sensitive to preincubation with prostaglandin E₁. This effect was apparently not due to elevated levels of cAMP since exogenous dibutyryl-cAMP did not alter the chlortetracycline fluorescence response to fMet—Leu—Phe. This is one of the few instances of prostaglandin E₁ affecting neutrophils at physiologic concentrations, dissociated from changes in cellular cyclic nucleotide levels

【 授权许可】

Unknown